| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 311 | Megasphaera massiliensis | AGCCGTACCTTTTCCGACG | TCACGTCCCGTTTGGCAA | 60.08 | 59.81 | 193 | 74 |
100.00%
|
100.00%
|
| Primer 312 | Megasphaera massiliensis | CAGTGCCGTTATGCTGGGA | CGGCAAATTGGCTGTCGAG | 60.08 | 59.86 | 176 | 73 |
100.00%
|
100.00%
|
| Primer 313 | Megasphaera massiliensis | ATGTCGCAACGTACGGCT | TCCCCAAAACCGCACCAT | 59.74 | 59.48 | 294 | 72 |
100.00%
|
100.00%
|
| Primer 314 | Megasphaera massiliensis | AGCCACGGCATCGAAAGAA | ACCATGACGCGATTGGCA | 60.00 | 60.05 | 288 | 72 |
100.00%
|
100.00%
|
| Primer 315 | Megasphaera massiliensis | AGCTGCTCATTCCCTGCT | ACCATGACGCGATTGGCA | 58.59 | 60.05 | 211 | 72 |
100.00%
|
100.00%
|
| Primer 316 | Megasphaera massiliensis | AAGCTGCTCATTCCCTGCT | ACCATGACGCGATTGGCA | 59.31 | 60.05 | 212 | 72 |
100.00%
|
100.00%
|
| Primer 317 | Megasphaera massiliensis | TCCACACCTTGCTGTGCA | TGCAGTCAACGGCATGGT | 59.41 | 59.89 | 222 | 72 |
100.00%
|
100.00%
|
| Primer 318 | Megasphaera massiliensis | TCCACACCTTGCTGTGCAT | TGCAGTCAACGGCATGGT | 59.85 | 59.89 | 222 | 72 |
100.00%
|
100.00%
|
| Primer 319 | Megasphaera massiliensis | CACCTTGCTGTGCATTGCA | TGCAGTCAACGGCATGGT | 59.64 | 59.89 | 218 | 72 |
100.00%
|
100.00%
|
| Primer 320 | Megasphaera massiliensis | ACGGCATCGAACGGGAAAT | ACCCGTTGCGCTTCATCA | 60.08 | 59.97 | 171 | 72 |
100.00%
|
100.00%
|